Journal
JOURNAL OF LEUKOCYTE BIOLOGY
Volume 80, Issue 2, Pages 433-447Publisher
FEDERATION AMER SOC EXP BIOL
DOI: 10.1189/jlb.0206112
Keywords
myeloid; transcription factor; myeloid leukemia; microarray; gene expression
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Funding
- NHLBI NIH HHS [R01 HL60657] Funding Source: Medline
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Mechanisms controlling the balance between proliferation and self-renewal versus growth suppression and differentiation during normal and leukemic myelopoiesis are not understood. We have used the bi-potent FDB1 myeloid cell line model, which is responsive to myelopoietic cytokines and activated mutants of the granulocyte macrophage-colony stimulating factor (GM-CSF) receptor, having differential signaling and leukemogenic activity. This model is suited to large-scale gene-profiling, and we have used a factorial time-course design to generate a substantial and powerful data set. Linear modeling was used to identify gene-expression changes associated with continued proliferation, differentiation, or leukemic receptor signaling. We focused on the changing transcription factor profile, defined a set of novel genes with potential to regulate myeloid growth and differentiation, and demonstrated that the FDB1 cell line model is responsive to forced expression of oncogenes identified in this study. We also identified gene-expression changes associated specifically with the leukemic GM-CSF receptor mutant, V449E. Signaling from this receptor mutant downregulates CCAAT/enhancer-binding protein ox (C/EBP alpha) target genes and generates changes characteristic of a specific acute myeloid leukemia signature, defined previously by gene-expression profiling and associated with C/EBP alpha mutations. J. Leukoc. Biol. 80: 433-447; 2006.
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