4.8 Article

Photoelectrochemical immunosensor for label-free detection and quantification of anti-cholera toxin antibody

Journal

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
Volume 128, Issue 30, Pages 9693-9698

Publisher

AMER CHEMICAL SOC
DOI: 10.1021/ja062729z

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We demonstrate herein a newly developed photoelectrochemical immunosensor for the determination of anti-cholera toxin antibody by using a photosensitive biotinylated polypyrrole film. The latter was generated by electro-oxidation of a biotinylated tris(bipyridyl) ruthenium(II) complex bearing pyrrole groups. The photoexcitation of this modified electrode potentiostated at 0.5 V vs SCE, in the presence of an oxidative quencher, pentaaminechloro cobalt(III) chloride (15 mM), led to a cathodic photocurrent. As a result of the affinity interactions, a layer of biotinylated cholera toxin was firmly bound to the functionalized polypyrrole film via avidin bridges. The resulting modified electrodes were tested as immunosensors for the detection of the corresponding antibody from 0 to 200 mu g mL(-1). The antibody concentration was measured through the decrease in photocurrent intensity resulting from its specific binding onto the polymeric coating, the detection limit being 0.5 mu g mL(-1).

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