Journal
FEBS LETTERS
Volume 580, Issue 18, Pages 4401-4408Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2006.07.005
Keywords
Wig-1; p53; dsRNA; zinc finger; siRNA; miRNA
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Wig-1 is a p53-induced zinc finger protein. Here we show that human Wig-1 binds long (>= 23 bp) dsRNAs with 5'-overhangs. The first zinc finger domain is necessary but not sufficient for this dsRNA-binding in vitro. Wig-1 also binds dsRNA in living cells via zinc fingers 1 and 2. Both zinc fingers 1 and 2 are important for Wig-1-mediated growth suppression. Moreover, Wig-1 binds 21 bp dsRNAs with 3'-protruding ends. These findings demonstrate that human Wig-1 can bind different types of dsRNAs, including dsRNAs resembling small interfering RNAs (siRNAs) and microRNAs (miRNAs), and indicate that dsRNA binding has a role in Wig-1-mediated regulation of cell growth. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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