Journal
JOURNAL OF MOLECULAR BIOLOGY
Volume 361, Issue 2, Pages 209-215Publisher
ACADEMIC PRESS LTD ELSEVIER SCIENCE LTD
DOI: 10.1016/j.jmb.2006.06.027
Keywords
polymorphisms; helical polymers; cryo-EM
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Funding
- NIBIB NIH HHS [EB001567] Funding Source: Medline
- NIGMS NIH HHS [GM035269, GM50776] Funding Source: Medline
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Many thin helical polymers, including bacterial pili and filamentous bacteriophage, have been seen as refractory to high-resolution studies by electron microscopy. Studies of the quaternary structure of such filaments have depended upon techniques such as modeling or X-ray fiber diffraction, given that direct visualization of the subunit organization has not been possible. We report the first image reconstruction of a filamentous virus, bacteriophage fd, by cryoelectron microscopy. Although these thin (similar to 70 angstrom in diameter) rather featureless filaments scatter weakly, we have been able to achieve a nominal resolution of similar to 8 A using an iterative helical reconstruction procedure. We show that two different conformations of the virus exist, and that in both states the subunits are packed differently than in conflicting models previously proposed on the basis of X-ray fiber diffraction or solid-state NMR studies. A significant fraction of the population of wild-type fd is either disordered or in multiple conformational states, while in the presence of the Y21M mutation, this heterogeneity is greatly reduced, consistent with previous observations. These results show that new computational approaches to helical reconstruction can greatly extend the ability to visualize heterogeneous protein polymers at a reasonably high resolution. (c) 2006 Elsevier Ltd. All rights reserved.
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