Journal
SCIENCE
Volume 313, Issue 5788, Pages 845-848Publisher
AMER ASSOC ADVANCEMENT SCIENCE
DOI: 10.1126/science.1128439
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The Arabidopsis CLAVATA3 (CLV3) gene encodes a stem cell - specific protein presumed to be a precursor of a secreted peptide hormone. Matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry (MALDI-TOF MS) applied to in situ Arabidopsis tissues determined the structure of a modified 12 - amino acid peptide (MCLV3), which was derived from a conserved motif in the CLV3 sequence. Synthetic MCLV3 induced shoot and root meristem consumption as cells differentiated into other organs, displaying the typical phenotype of transgenic plants overexpressing CLV3. These results suggest that the functional peptide of CLV3 is MCLV3.
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