Journal
ANALYTICA CHIMICA ACTA
Volume 575, Issue 2, Pages 217-222Publisher
ELSEVIER
DOI: 10.1016/j.aca.2006.05.097
Keywords
spiro form fluorescein hydrazide; fluorescence-on reaction; copper; selective detection
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The spiro form fluorescein hydrazide (1) bearing a cleavable active bond is characterized as a highly selective and sensitive fluorescence probe for Cu2+. In 0.01 M Tris-HCl buffer (pH 7.2), the probe displays a highly selective fluorescence-on response to Cu2+ only, instead of other common metal ions. The reaction mechanism has been explored and proposed as that the hydrazide group of 1 recognizes and binds Cu2+, and the subsequent complexation of Cu2+ promotes hydrolytic cleavage of the amide bond, causing the release of fluorophore (fluorescein) and thereby the retrievement of fluorescence. Conditions for fluorescence measurement were optimized, generating a calibration curve that is linear over the concentration range of 0.1-10 mu M Cu2+. The detection limit for Cu2+, is 64 nM based on 11 blank determinations (k = 3), also showing a highly sensitive feature. The fluorescence-on reaction described here has been used for the determination of trace Cu2+ in real biological fluids with satisfactory results. (c) 2006 Elsevier B.V. All rights reserved.
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