Journal
PLASMA PROCESSES AND POLYMERS
Volume 3, Issue 6-7, Pages 498-505Publisher
WILEY-V C H VERLAG GMBH
DOI: 10.1002/ppap.200600010
Keywords
biopolymers; di(ethylene glycol) mono vinyl ether (EO2); plasma enhanced chemical vapor deposition (PECVD); polymerization; surface plasmon enhanced fluorescence spectroscopy (SPEFS)
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We report on the development of a multilayer system, which allows for specific DNA binding reactions to occur at the surface, but at the same time shows antifouling properties, thus minimizing the nonspecific adsorption of proteins (BSA). The synthesis involves the combination of plasma polymerization and streptavidin-biotin assembly. DNA sensing is achieved using SPFS. Antifouling properties are tailored by controlling the film thickness, together with the effects of the swelling in buffer solution and by the specific surface chemical design. Preliminary hybridization experiments show promising results.
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