Journal
BIOORGANIC & MEDICINAL CHEMISTRY
Volume 14, Issue 16, Pages 5439-5445Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.bmc.2006.05.012
Keywords
alkaloid; DNA-binding; intercalations; fluorescence spectrometry; sequence selectivity
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A comparative study on the intercalating binding of sanguinarine, chelerythrine, and nitidine with CT DNA, poly(dG-dC)(.)poly(dG-dC), poly(dA-dT)(.)poly(dA-dT), and seven sequence-designed double-stranded oligodeoxynucleotides has been performed using fluorometric and spectrophotometric techniques, aiming at providing insights into their sequence selectivity for DNA-binding. The results show that both sanguinarine and nitidine bind preferentially to DNA containing alternating GC base pairs [d(TGCGCA)(2)], while chelerythrine exhibits quite distinct sequence selectivity from sanguinarine, which shows a high specificity for DNA containing contiguous GC base pairs [5 '-TGGGGA-3 '/3 '-ACCCCT-5 ']. (c) 2006 Elsevier Ltd. All rights reserved.
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