Synthesis, characterization of biodegradable dextran-allyl isocyanate-ethylamine/polyethylene glycol-diacrylate hydrogels and their in vitro release of albumin

Based on dextran-allyl isocyanate-ethylamine (Dex-AE) and polyethylene glycol-diacrylate (PEGDA), a pH sensitive biodegradable hydrogel with improved protein release was prepared through UV photo-crosslinking. The Dex-AE precursor was prepared through a two-step chemical modification and characterized by FT-IR and H-1 NMR. The effects of reaction conditions on the synthesis of Dex-AE were studied. The interior morphology data by scanning electron microscopy (SEM) revealed that an increase in Dex-AE content led to an initial decrease in pore size of the microstructure of the Dex-AE/PEGDA hydrogels, but a further increase in Dex-AE content resulted in a relatively looser network structure. The swelling data indicated that the swelling ratio depended on the precursor feed ratio and was correlated to the morphology of the microporous network structure. The pH sensitive property of the Dex-AE/PEGDA hydrogels was studied in different pH buffer solutions and was found that these hydrogels were pH sensitive due to the presence of ample pendant amino groups. The ionic strength data demonstrated that the Dex-AE/PEGDA hydrogels exhibit the highest swelling ratio in pure water, but the swelling ratio became lower as the ionic strength of the media increased. The in vitro albumin release from these hydrogels was examined in different pH (3.0, 7.4) buffer solutions. Both the protein loading and release data indicated that the Dex-AE/PEGDA hydrogels had more protein loading and sustained release capability than pure PEGDA hydrogel, and this ability increased as the Dex-AE composition increased; the Dex-AE/PEGDA hydrogel also showed a faster BSA release in a lower pH than a higher pH medium. (c) 2006 Published by Elsevier Ltd.