Journal
TALANTA
Volume 70, Issue 1, Pages 153-159Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.talanta.2005.11.049
Keywords
tetracycline antibiotics; fish; in-tube SPME-HPLC; poly (methacrylic acid-ethylene glycol dimethacrylate) monolithic capillary
Categories
Ask authors/readers for more resources
An on-line simple and rapid method for the simultaneous determination of tetracycline (TC), oxytetracycline (OTC), chlortetracycline (CTC) and doxycycline (DC) residues in fish muscle was developed by coupling in-tube solid-phase microextraction (SPME) to high-performance liquid chromatography (HPLC) with a photodiode array detector. Biocompatible poly (methacrylic acid-ethylene glycol dimethacrylate) monolithic capillary was selected as the extraction medium, and no precipitating protein and removing fat steps were required prior to extraction. In order to optimize the extraction of these compounds, several in-tube SPME parameters were investigated. Simply performed by extracting with 0.01 M EDTA-MacIlvaine buffer solution (pH 4.0) and centrifugation, the sample then could be directly injected into the device for extraction. The limits of detection of tetracycline, oxytetracycline, chlortetracycline and doxycycline were calculated to be 22, 16, 30 and 21 ng/g, respectively. The calibration curves showed linearity in the range of 100-10,000ng/g with a linear coefficient R-2 value above 0.9980. Excellent method ng/g reproducibility was found by intra- and inter-day precisions, yielding the R.S.D.s less than 4.22% and 5.71%, respectively. (c) 2005 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available