Journal
NEUROREPORT
Volume 17, Issue 12, Pages 1331-1335Publisher
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1097/01.wnr.0000230515.86090.bc
Keywords
gamma-aminobutyric acid type A receptor; mouse; patch clamp; reverse-transcriptase polymerase chain reaction; substantia gelatinosa; tonic current
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We used whole-cell recording to identify, for the first time, GABAergic tonic current in subpopulations of substantia gelatinosa neurons in mouse spinal cord slices. Application of the gamma-aminobutyric acid type A receptor antagonist bicuculline revealed tonic currents in 49 of 73 substantia gelatinosa neurons. These tonic currents were dissected into three groups according to their diverse pharmacological properties. In the first group, furosemide failed to block the tonic current while miclazolam and propofol potentiated it. In the second group, furosemide blocked the tonic current but miclazolam and propofol failed to enhance the current. In the third group, furosemide blocked and 3 alpha, 21-dihydroxy-5 alpha-pregnan-20-one enhanced the tonic current. The presence of mRNAs for alpha(4), alpha(5), alpha(6) delta and epsilon subunits in the substantia gelatinosa (reverse-transcriptase polymerase chain reaction) suggest that gamma-aminobutyric acid type A receptors comprising alpha(5)beta(x)gamma(2), alpha(x)beta(x)epsilon and alpha(4)beta(x)delta subunits are responsible for tonic currents.
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