Journal
FEBS LETTERS
Volume 580, Issue 19, Pages 4703-4708Publisher
WILEY
DOI: 10.1016/j.febslet.2006.07.054
Keywords
copper complexes; green tea polyphenols; Ribonuclease A; noncompetitive inhibition; Fourier transformed infrared; circular dichroism
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Green tea polyphenols, which have the ability to inhibit angiogenesis, form complexes with Cu(II), a known potent stimulator of blood vessel proliferation. Copper complexes of (-)-epicatechin gallate and (-)-epigallocatechin gallate were found to inhibit the enzymatic activity of Ribonuclease A (RNase A) as revealed by an agarose gel based assay and urea denatured gel electrophoresis. The copper complexes were found to be noncompetitive inhibitors of RNase A with inhibition constants in the micromolar range. Changes in the secondary structure of the protein are found to occur due to the interaction as revealed from Fourier transform infrared and circular dichroism studies. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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