Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 103, Issue 34, Pages 12819-12824Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0605778103
Keywords
DNA repair; recombination; yeast
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Funding
- NIGMS NIH HHS [GM24110, R01 GM024110] Funding Source: Medline
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We developed a system that allows the selection of the reciprocal products resulting from spontaneous mitotic cross-overs in the yeast Saccharomyces cerevisiae. A number of other types of genetic events, including chromosome loss, can be monitored with this system. For a 120-kb chromosome interval on chromosome V (CEN5-CAN1), the rate of mitotic cross-overs was 4 x 10(-5) per division, a rate approximate to 25,000-fold lower than the meiotic rate of crossovers. We found no suppression of mitotic cross-overs near the centromere of chromosome V, unlike the suppression observed for meiotic exchanges. The rate of reciprocal cross-overs was substantially (38-fold) elevated by treatment of cells with hydroxyurea, a drug that reduces nucleotide pools and slows DNA replication.
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