Journal
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
Volume 182, Issue 2, Pages 158-167Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotochem.2006.02.004
Keywords
guajacol; human serum albumin; molecule modeling; Fourier transform infrared (FT-IR) spectroscopy; secondary structure; circular dichroism (CD); fluorescence anisotropy
Categories
Ask authors/readers for more resources
The fluorogenic property of guaiacol was exploited for the first time to analyze the interaction with target protein as a probe by molecule modeling, fluorescence, Fourier transform infrared spectroscopy (Fr-IR), and circular dichroism (CD) spectroscopy. Molecular docking was 14 performed to reveal the possible binding mode or mechanism, suggested that guaiacol can strongly bind to human serum albumin (HSA) and the primary binding site of guaiacol is located in site I of HSA. It is considered that guaiacol binds to site I (subdomain 11) mainly by a hydrophobic interaction and there is a hydrogen bond interactions between the drug and the residues Ala 291, which is good agreement with the results from the experimental thermodynamic parameters (the enthalpy change Delta H degrees and the entropy change AS' were calculated to be 104.008 KJ/mol and 0.409J/mol K according to the van't Hoff equation). Data obtained by the fluorescence spectroscopy indicated that binding of guaiacol with HSA leads to dramatic enhancement in the fluorescence emission intensity along with significant occurrence of efficient Forster type resonance energy transfer (FRET) from HSA to the protein bound guaiacol. From the high value of fluorescence anisotropy (r=0.40) it is argued that the probe molecule is located in motionally restricted environment of the protein. The binding constants for the interaction of guaiacol with HSA have been A evaluated from relevant fluorescence data. The alterations of protein secondary structure in the presence of guaiacol in aqueous solution were quantitatively calculated by the evidences from FT-IR and CD spectroscopes. (c) 2006 Elsevier B.V. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available