Journal
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
Volume 102, Issue 3, Pages 233-236Publisher
SOC BIOSCIENCE BIOENGINEERING JAPAN
DOI: 10.1263/jbb.102.233
Keywords
poly(3-hydroxybutyrate); Corynebacterium glutamicum; biodegradable plastic; biopolyester
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A biosynthetic pathway for poly(3-hydroxybutyrate) [P(3HB)] production by Corynebacterium glutamicum was developed by introducing the phbCAB operon derived from Ralstonia eutropha. P(3HB) synthase activity was detected in this recombinant C glutamicum carrying a cell surface protein gene promoter. Intracellular P(3HB) was microscopically observed as inclusion granules and its content was calculated to be 22.5% (w/w) with a number average molecular weight of 2.1 x 10(5) and a polydispersity of 1.63.
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