Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 11, Issue 5, Pages -Publisher
SPIE-INT SOCIETY OPTICAL ENGINEERING
DOI: 10.1117/1.2357735
Keywords
sperm; tracking; motility; automation; CASA
Funding
- NCRR NIH HHS [RR 14892] Funding Source: Medline
Ask authors/readers for more resources
This paper describes a robust single sperm tracking algorithm (SSTA) that can be used in laser optical trapping and sperm motility studies. The algorithm creates a region of interest (ROI) centered about a sperm selected by the user. SSTA contrast enhances the ROI image and implements a modified four-class thresholding method to extract the tracked sperm as it transitions in and out of focus. The nearest neighbor method is complemented with a speed-check feature to aid tracking in the presence of additional sperm or other particles. SSTA has a collision-detection feature for real or perceived collision or near-miss cases between two sperm. Subsequent postcollision analysis employs three criteria to distinguish the tracked sperm in the image. The efficacy of SSTA is validated through examples and comparisons to commercially available computer-aided sperm tracking systems. (c) 2006 Society of Photo-Optical Instrumentation Engineers.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available