Journal
JOURNAL OF MASS SPECTROMETRY
Volume 41, Issue 9, Pages 1171-1178Publisher
WILEY
DOI: 10.1002/jms.1082
Keywords
tandem mass spectrometry; metabolite identification; drug metabolism; sibutramine; carbamoyl glucuronides
Ask authors/readers for more resources
We present a detailed experimental approach to detection and subsequent structural characterization of unknown metabolites of sibutramine, using liquid chromatography-mass spectrometric techniques. The full, precursor ion, and constant neutral loss scan modes of a triple quadrupole mass spectrometer were used for screening sibutramine metabolites in human urine. The structural assessment of unknown metabolites was based on MS ion trap mass spectrometric analysis and comparison of MS spectra between the standards and compounds detected. Two phase-I (M1 and M2) and eight phase-II (M3-M6) metabolites of sibutramine were found in human urine. Metabolites M1 and M2, which were found as minor metabolites, originated from N-demethylation of sibutramine. Carbamoyl glucuronides. formed from metabolites M1, M2, and their hydroxylated analogs were the main metabolites of sibutramine and wen! characterized by tandem mass spectrometric analysis and by the chemical modification of their structure. We demonstrate the usefulness of the chemical derivatization approach for estimation of the site of glucuronidation and propose the formation of hydroxylated regioisomers of metabolites M4 and M6. Copyright (c) 2006 John Wiley & Sons, Ltd.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available