4.6 Article

Non-genomic immunosuppressive actions of progesterone inhibits PHA-induced alkalinization and activation in T cells

Journal

JOURNAL OF CELLULAR BIOCHEMISTRY
Volume 99, Issue 1, Pages 292-304

Publisher

WILEY
DOI: 10.1002/jcb.20858

Keywords

progesterone; non-genomic; immunosuppressive; Ca2+; pH(i); IL-2; IL-4; proliferation; T cells

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Progesterone is an endogenous immunomodulator, and can suppress-F-cell activation during pregnancy. When analyzed under a genome time scale, the classic steroid receptor pathway does not have any effect on ion fluxes. Therefore, the aim of this study was to investigate whether the non-genomic effects on ion fluxes by progesterone could immunosuppress phytohemagglutinin (PHA)-induced human peripheral T-cell activation. The new findings indicated that, first, only progesterone stimulated both [Ca2+](i) elevation and pH(i) decrease; in contrast, estradiol or testosterone stimulated [Ca2+](i) elevation and hydrocortisone or dexamethasone stimulated pHi decrease. Secondly, the [Ca2+](i) increase by progesterone was dependent on Ca2+ influx, and the acidification was blocked by Na+/H+ exchange (NHE) inhibitor, 3-methylsulphonyl-4-piperidinobenzoyl, guanidine hydrochloride (HOE-694) but not by 5-(N,N-dimethyl)amiloride (DMA). Thirdly, progesterone blocked phorbol 12-myristate 13-acetate (PMA) or PHA-induced alkalinization, but PHA did not prevent progesterone-induced acidification. Fourthly, progesterone did not induce T-cell proliferation; however, co-stimulation progesterone with PHA was able to suppress PHA-induced IL-2 or IL-4 secretion and proliferation. When progesterone was applied 72 h after PHA stimulation, progesterone could suppress PHA-induced T-cell proliferation. Finally, immobilization of progesterone by conjugation to a large carrier molecule (BSA) also stimulated a rapid [Ca2+](i) elevation, pH(i) decrease, and suppressed PHA-induced proliferation. These results suggested that the nongenomic effects of progesterone, especially acidification, are exerted via plasma membrane sites and suppress the genomic responses to PHA. Progesterone might act directly through membrane specific nonclassical steroid receptors to cause immunomodulation and suppression of T-cell activation during pregnancy.

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