Journal
JOURNAL OF PLANT RESEARCH
Volume 119, Issue 5, Pages 469-478Publisher
SPRINGER JAPAN KK
DOI: 10.1007/s10265-006-0009-4
Keywords
berry ripening; cold stress; grapevine; V-PPase
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Gene expression of grapevine vacuolar H+-pyrophosphatase (V-PPase EC 3.6.1.1.) during fruit ripening has previously been reported. Here we report on putative multiple V-PPase isoforms in grapevine. In this study a full-length cDNA sequence with an open reading frame of 2,295 nucleotides encoding a V-PPase gene (vpp2: acc. nr. AJ557256) was cloned. Sequence analyses of the deduced amino acid residues and RTPCR experiments indicated that Vitis vinifera L. has at least two distinct isoforms of the V-PPase gene. Bioinformatic analyses of 13 V-PPase protein sequences revealed two highly conserved motifs associated with pyrophosphate (PPi) binding and response to stress, respectively. Both V-PPase isoforms were expressed at higher levels in the late post-veraison stage of grape berry ripening. Results also showed that the expression of grapevine V-PPase was induced by cold stress.
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