Journal
JOURNAL OF PROTEOME RESEARCH
Volume 5, Issue 9, Pages 2438-2447Publisher
AMER CHEMICAL SOC
DOI: 10.1021/pr060186m
Keywords
glycoproteome; diagonal chromatography; COFRADIC; PNGase F
Categories
Ask authors/readers for more resources
A procedure to map N-glycosylation sites is presented here. It can be applied to purified proteins as well as to highly complex mixtures. The method exploits deglycosylation by PNGase F in a diagonal, reverse-phase chromatographic setup. When applied to 10 mu L of mouse serum, affinity-depleted for its three most abundant components, 117 known or predicted sites were mapped in addition to 10 novel sites. Several sites were detected on soluble membrane or receptor components. Our method furthermore senses the nature of glycan structures and can detect differential glycosylation on a given site. These properties-high sensitivity and dependence on glycan imprinting-can be exploited for glycan-biomarker analysis.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available