Journal
MICROBIOLOGY-SGM
Volume 152, Issue -, Pages 2749-2756Publisher
SOC GENERAL MICROBIOLOGY
DOI: 10.1099/mic.0.28924-0
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- NIAID NIH HHS [AI36973, AI37856, AI43846, AI51668] Funding Source: Medline
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The wbl (whiB-like) genes encode putative transcription factors unique to actinomycetes. This study characterized the promoter element of one of the seven wbl genes of Mycobacterium tuberculosis, whiB 1 (Rv3219c). The results reveal that whiB 1 is transcribed by a class I-type cAMP receptor protein (CRP)-dependent promoter, harbouring a CRIP-binding site positioned at -58.5 with respect to its transcription start point. In vivo promoter activity analysis and electrophoretic mobility shift assays suggest that the expression of whiB1 is indeed regulated by cAMP-dependent binding of CRPM (encoded by the M. tuberculosis gene Rv3676) to the whiB1 5' untranslated region (5'UTR). beta-Galactosidase gene fusion analysis revealed induction of the whiB1 promoter in M. tuberculosis on addition of exogenous dibutyric cAMP (a diffusible cAMP analogue) only when an intact CRIP-binding site was present. These results indicate that M. tuberculosis whiB1 transcription is regulated in part by cAMP levels via direct binding of cAMP-activated CRPM to a consensus CRP-binding site in the whiB1 5'UTR.
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