Journal
SOIL BIOLOGY & BIOCHEMISTRY
Volume 38, Issue 9, Pages 2963-2966Publisher
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/j.soilbio.2006.03.019
Keywords
cellulases; compost; polyphenol oxidases; thermophiles
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In composting, organic matter is degraded by cellulases and ligninolytic enzymes at temperatures typically above 50 degrees C. This study isolated thermophilic microorganisms from a compost system that were then screened for cellulase and polyphenol oxidase activity. Temperature optima for the cellulases and polyphenol oxidases were determined as 70 and 40 degrees C, respectively. Maximal cellulase activity was determined as 1.333 mg glucose released ml(-1) min(-1). Maximal polyphenol oxidase activity attained was 5.111 nmol phenol ml(-1) min(-1). Cellulases were found to be stable over a period of 1 h. The isolated compost microorganisms were identified as strains of Bacillus using 16S ribosomal DNA sequence analysis. (c) 2006 Elsevier Ltd. All rights reserved.
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