Journal
PLASMID
Volume 56, Issue 2, Pages 133-137Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.plasmid.2006.03.002
Keywords
Moraxella catarrhalis; plasmid; complementation
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Funding
- NIAID NIH HHS [AI36344] Funding Source: Medline
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The plasmid shuttle vector pWW102B is able to replicate in only a modest number of Moraxella catarrhalis strains. Plasmid pWW115, a spontaneous deletion mutant of pWW102B, was shown to lack both the pACYC184-derived origin of replication and the associated chloramphenicol-resistance gene but was able to replicate in every M. catarrhalis strain tested in this study, including one strain that had been previously refractory to all types of genetic manipulations. To test the utility of this plasmid, a M. catarrhalis gene encoding the UspA2 serum-resistance factor was cloned into pWW115 and the resultant recombinant plasmid was shown to confer serum-resistance on a serum-sensitive M. catart-halis uspA2 mutant. (c) 2006 Elsevier Inc. All rights reserved.
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