Journal
LICHENOLOGIST
Volume 38, Issue -, Pages 435-440Publisher
CAMBRIDGE UNIV PRESS
DOI: 10.1017/S0024282906005779
Keywords
actin; algae; Asterochloris; ITS rDNA; lichen; phylogenetic analyses
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Primers are presented to amplify an intron-containing portion of the actin type I locus from Asterochloris algae. The amount of variation and phylogenetic resolution provided by the ITS rDNA are compared with that from the actin locus. The number of variable and parsimony-informative sites within the actin locus was drastically higher than that of the ITS. Phylogenetic analyses of the ITS resulted in poorly resolved trees with low support values. In contrast, phylogenetic analyses of the actin locus yielded trees with better support and resolution. It is concluded that the actin type I introns will be useful for studies investigating species concepts and population genetics.
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