Journal
FUNGAL GENETICS AND BIOLOGY
Volume 43, Issue 9, Pages 630-641Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.fgb.2006.04.001
Keywords
nitrogen deficiency; starvation stress; gene expression; microarrays; truffles
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The focus of this work is on the nitrogen starvation stress responses operating in a plant symbiotic fungus. A cDNA array profiling analysis was conducted on N-limited mycelia of the mycorrhizal ascomycete Tuber borchii. Fifty-one unique transcripts, out of 2062 redundant arrayed cDNAs, were differentially expressed by at least 1.5-fold in response to N deprivation. Only two N assimilation components-a nitrate transporter and a high-affinity ammonium transporter' were found among differentially expressed genes. All the other N status responsive genes code for as yet unidentified hypothetical proteins or components not directly involved in N assimilation or metabolism, especially carbohydrate binding proteins and oligosaccharide as well as lipid modifying enzymes. A subset of cDNA array data were confirmed and extended by Northern blot analysis, which showed that most of the. latter components respond not only to nitrogen, but also to carbon source depletion. (c) 2006 Elsevier Inc. All rights reserved.
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