Journal
JOURNAL OF BIOMEDICAL OPTICS
Volume 11, Issue 5, Pages -Publisher
SPIE-INT SOCIETY OPTICAL ENGINEERING
DOI: 10.1117/1.2363363
Keywords
laser scanning confocal microscopy; reflectance imaging; heterodyne detection; dysplasia; endoscopy; in vivo imaging
Funding
- NCI NIH HHS [K08 CA096752, 5U54CA105296, U54 CA105296] Funding Source: Medline
- NIDDK NIH HHS [K08 DK067618, K08 DK067618-04, K08 DK067618-05] Funding Source: Medline
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A dual-axes confocal reflectance microscope has been developed that utilizes a narrowband laser at 1310 nm to achieve high axial resolution, image contrast, field of view, and tissue penetration for distinguishing among normal, hyperplastic, and dysplastic colonic mucosa ex vivo. Light is collected off-axis using a low numerical aperture objective to obtain vertical image sections, with 4- to 5-mu m resolution, at tissue depths up to 610 mu m. Post- objective scanning enables a large field of view (610 x 640 mu m), and balanced-heterodyne detection provides sensitivity to collect vertical sections at one frame per second. System optics are optimized to effectively reject out-of-focus scattered light without use of a low-coherence gate. This design is scalable to millimeter dimensions, and the results demonstrate the potential for a miniature instrument to detect precancerous tissues, and hence to perform in vivo histopathology. (c) 2006 Society of Photo-Optical Instrumentation Engineers.
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