Journal
ENDOCRINOLOGY
Volume 147, Issue 9, Pages 4303-4310Publisher
ENDOCRINE SOC
DOI: 10.1210/en.2006-0397
Keywords
-
Categories
Ask authors/readers for more resources
Estrogen receptor-dependent organizational events between birth [ postnatal day ( PND) 0] and PND 14 affect development and function of porcine uterine tissues. Observations that uterotrophic effects of relaxin (RLX) in neonatal gilts were inhibited by the antiestrogen ICI 182,780 suggested that a RLX signaling system, capable of cross-talk with the estrogen receptor, evolves during a critical period for uterine programming ( PND 0 - 14). Objectives were to determine 1) effects of age and estrogen exposure from birth on porcine uterine RLX/insulin-like 3 receptor (LGR7/LGR8) expression and 2) whether milk serves as a natural source of RLX in neonatal pigs. Uterine LGR7/LGR8 expression, detected by RT-PCR and in situ hybridization on PND 0, 7, and 14, was predominantly stromal for LGR7, myometrial for LGR8, and increased with age and after treatment with estradiol valerate ( 50 mu g/kg body weight (.) d) from birth. Stromal expression of LGR7 was also detected immunohistochemically. Milk RLX concentrations declined ( P < 0.001) from 17.3 +/- 1.4 ng/ml ( lactation d 0) to 1.7 +/- 0.3 ng/ml ( lactation d 14). RLX, present in the serum of nursing pigs on PND 0 and 1, was undetectable before nursing and in neonates fed RLX-free milk replacer for 12 h. Thus, a developmentally regulated, estrogen-sensitive LGR7 and LGR8 receptor system is present in the porcine uterus at birth and may be activated by milk-borne RLX delivered into the circulation during the first 48 h of postnatal life. Maternal lactocrine contributions to the neonatal hormonal milieu could affect the developmental programming of uterine and other somatic tissues.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available