Journal
FERTILITY AND STERILITY
Volume 86, Issue 3, Pages 572-576Publisher
ELSEVIER SCIENCE INC
DOI: 10.1016/j.fertnstert.2006.02.090
Keywords
oocyte IVM; three-dimensional coculture; alginate; granulosa
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Objective: To verify the hypothesis that a three-dimensional, follicle-mimicking structure enhances in vitro maturation yields without hormonal supplementation in an in vitro maturation program. Design: Feasibility study; 204 anonymous-denuded,germinal vesicles retrieved from gonadotropin-treated women were cultured for 48 hours without hormonal supplementation in microdrop culture or in a three-dimemsional coculture with grantlosa cells in a barium alginate in membrane. Setting: An assisted reproduction center in Italy. Patient(s): One hundred twenty-two informed women. Intervention(s): Germinal vesicles retrieved after ovarian stimulation were denuded and cultured without hormonal supplementation in microdrop culture or in three-dimensional coculture with granulosa cells enclosed in the core of a barium alginate capsule. Main Outcome Measure(s): Oocytematuration and morphological investigation of follicle-mimicking structures. Result(s): Statistically significantly higher oocytematuration yields were obtained by using the three-dimensional, coculture system enclosed in a barium alginate membrane (after 48 hours: coculture, 90.3%; microdrop, 52.0%; odds ratio, 8.51). Morphological investigation indicated that after 48 hours of coculture, the decumulated oocyte appeared to be surrounded by a pseudocumulus structure that lies on the inner surface of the alginate membrane and protrudes toward the capsule's lumen. Conclusion(s): The maturation yield of cocultured oocytes is eightfold higher than that achieved with a microdrop maturation technique, making a higher number of gametes available for IVF programs. Stereomicroscopic morphological survey indicates a reassembly of granulosa cells surrounding oocyte zona, mimicking an antral cumulus oophorus. cumulus oophorus.
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