Journal
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS
Volume 26, Issue 8, Pages 1333-1337Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.msec.2005.08.028
Keywords
calcium; phosphate; biomineralization; SaOS-2; SHS; multiphasic
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The work presented here examines the effect of multiphasic CaP materials on the activity of SaOS-2 osteoblast like cells. Heterogeneous calcium phosphate (HCaP) was synthesized using self-propagating high temperature combustion synthesis (SHS). All samples were characterized using XRD, SEM, and FTIR. Cell culture procedures of HCaP with SaOS-2 cells followed ASTM 813-01. Samples were examined by Environmental SEM (ESEM) and EDS to characterize the mineral content of the cultures. Gold-coated samples were used for high-resolution imaging. Biomineralization was noted in unconditioned cultures exposed to HCaP while the controls (cells only in media and HCaP only in media) showed no mineralization. Calcium phosphate plate-like structures were identified adjacent to cells expressing calcium phosphate containing vesicles. These structures are similar to the organophosphate crystals seen in other published works. Plate-like crystals were noted in the larger membrane vesicles of SaOS-2 cells indicating the occurrence of cellularly mediated biomineralization. (c) 2005 Elsevier B.V. All rights reserved.
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