Journal
EUROPEAN JOURNAL OF CARDIO-THORACIC SURGERY
Volume 30, Issue 3, Pages 492-498Publisher
OXFORD UNIV PRESS INC
DOI: 10.1016/j.ejcts.2006.05.031
Keywords
hypothermic circulatory arrest; selective cerebral perfusion; perfusion temperature; cerebral protection; animal model
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Objective: Selective antegrade cerebral perfusion (SACP) seems to be associated with a better outcome compared to hypothermic circulatory arrest (HCA) alone. This study was undertaken to evaluate the influence of different SACP temperatures on the neurological integrity. Methods: Twenty-six pigs were included in the study and assigned to 100 min HCA at 20 degrees C body temperature without (n = 6) or with either 10 degrees C (n = 6), 20 degrees C (n = 7) or 30 degrees C (n = 7) of SACR Haemodynamics, metabolics and neurophysiology (EEG, SSEP, ICP, sagittal sinus saturation) were monitored. Animals were sacrified 4 h after reperfusion and brains perfused for histological and molecular genetic assessment. Results: There were no clinically relevant differences in haemodynamics between groups. The rise in ICP during SACP was significantly more marked in the 30 degrees C group (p < 0.05) and remained high during the entire experiment. In the 10 degrees C group the rise in ICP was postponed, but increased during reperfusion. The 20 degrees C group showed a slight increase of ICP over time, but remained significantly tower compared to HCA (p < 0.05). Sagittal sinus saturation decreased during SACP at 30 oC (p < 0.05). EEG recovery was most complete in the 20 degrees C group (p < 0.05). RT-PCR analysis of brain tissue revealed a reduction for heat shock protein (HSP-72) in 20 degrees C (p < 0.05) and 10 degrees C animals (p = 0.095). Histopathological. evaluation showed a reduction of edema and eosinophilic cells in the groups treated with SACR Conclusion: In this model, SACP is superior to HCA atone. Regarding the optimal temperature for SACP, it seems that 20 degrees C provides adequate brain protection in comparison to the potential detrimental effects of moderate (30 degrees C) and profound (10 degrees C) temperatures. (c) 2006 Elsevier B.V. All rights reserved.
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