Journal
FEBS LETTERS
Volume 580, Issue 20, Pages 4895-4899Publisher
WILEY
DOI: 10.1016/j.febslet.2006.08.005
Keywords
allergen; honeybee; IgE; hymenoptera; icarapin; prokaryotic expression; venom
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The 1045 bp full-length cDNA sequence of a new bee venom component was obtained by rapid amplification of cDNA ends. The 672 bp coding sequence corresponds to a protein with a signal peptide and multiple carbohydrate binding sites, and it was named icarapin. It has the new consensus sequence N-[TS]-T-S-[TV]-x-K-[VI](2)-[DN]-G-H-x-V-x-I-N-[ED]-T-x-Y-x-[DHK]- x(2,6)- [STA]-[VLFI]-x-[KR]-V-R-[VLI]-[IV]-[DN]-V-x-P. At least two transcript variants were found. Recombinant icarapin was tested for recognition by IgE antibodies and gave a positive dot blot with sera from 4 out of 5 bee venom allergic patients, all beekeepers. Indirect immunofluorescent staining localized the protein in the cuticular lining of the venom duct. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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