Journal
FEBS LETTERS
Volume 580, Issue 20, Pages 4764-4770Publisher
WILEY
DOI: 10.1016/j.febslet.2006.07.068
Keywords
phosphoproteomics; phosphorylation; bioinformatics; mass spectrometry
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Protein phosphorylation is important for regulation of most biological functions and up to 50% of all proteins are thought to be modified by protein kinases. Increased knowledge about potential phosphorylation of a protein may increase our understanding of the molecular processes in which it takes part. Despite the importance of protein phosphorylation, identification of phosphoproteins and localization of phosphorylation sites is still a major challenge in proteomics. However, high-throughput methods for identification of phosphoproteins are being developed, in particular within the fields of bioinformatics and mass spectrometry. In this review, we present a toolbox of current technology applied in phosphoproteomics including computational prediction, chemical approaches and mass spectrometry-based analysis, and propose an integrated strategy for experimental phosphoproteomics. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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