Journal
BIOTECHNOLOGY AND BIOENGINEERING
Volume 95, Issue 1, Pages 15-21Publisher
WILEY
DOI: 10.1002/bit.20930
Keywords
oxygen transfer; shake flask; closures; microbial growth; modeling
Categories
Ask authors/readers for more resources
Oxygen mass transfer in shake flasks is an important aspect limiting the culture of aerobic microorganisms. In this work, mass transfer of oxygen through a closure and headspace of shake flasks is investigated. New equations for prediction of k(G)a in shake flasks with closures are introduced. Using Pseudomonas putida, microbial growth on glucose (fast metabolism) and phenol (slow metabolism) in shake flasks with closures were studied, considering both substrate and oxygen restrictions. A combined model for oxygen mass transfer and microbial growth is shown to accurately predict experimental oxygen concentrations and oxygen yield factors during growth experiments more accurately than previous models. (c) 2006 Wiley Periodicals, Inc.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available