Journal
EMBO JOURNAL
Volume 25, Issue 17, Pages 4097-4107Publisher
WILEY
DOI: 10.1038/sj.emboj.7601289
Keywords
dendritic cells; integrin; mindin; rho GTPases
Categories
Funding
- NIAID NIH HHS [AI61364, AI54685, U01 AI061364] Funding Source: Medline
Ask authors/readers for more resources
Rho guanosine triphosphatases (GTPases) regulate multiple aspects of dendritic cell (DC) function, but what regulates the expression of Rho GTPases in DCs is unknown. Here, we show that the extracellular matrix protein mindin regulates the expression of Rho GTPases in DCs. Mindin(-/-) mice displayed defective CD4+ T-cell priming and impaired humoral immune responses to T-dependent antigens. Mindin(-/-) DCs had reduced expression of Rac1/2 and impaired priming capacity owing to inefficient engagement with T lymphocytes. Ectopic Rac1 expression restored the priming capability of Mindin(-/-) DCs. Furthermore, we show that DC adhesion to mindin matrix was blocked by antibodies to alpha(4), alpha(5) and beta(1) integrins. DCs lacking beta(1) integrin had reduced adhesion to mindin matrix, decreased expression of Rac1/2 and impaired priming capacity. These results suggest that mindin-integrin interactions play a key role in regulating Rho GTPase expression in DCs and DC priming of T lymphocytes.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available