Journal
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
Volume 103, Issue 37, Pages 13635-13639Publisher
NATL ACAD SCIENCES
DOI: 10.1073/pnas.0606294103
Keywords
protein design; Puf proteins; protein-RNA interaction; adenosine-uracil-rich elements
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Funding
- Intramural NIH HHS Funding Source: Medline
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Puf proteins bind RNA sequence specifically and regulate translation and stability of target mRNAs. A code for RNA recognition has been deduced from crystal structures of the Puf protein, human Pumilio1, where each of eight repeats binds an RNA base via a combination of three side chains at conserved positions. Here, we report the creation of seven soluble mutant proteins with predictably altered sequence specificity, including one that binds tightly to adenosine-uracil-rich element RNA. These data show that Pumilio1 can be used as a scaffold to engineer RNA-binding proteins with designed sequence specificity.
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