Journal
DEVELOPMENTAL BIOLOGY
Volume 297, Issue 2, Pages 493-507Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.ydbio.2006.05.027
Keywords
posterior prevalence; HOXD13; Hox; homeodomain; limb
Categories
Funding
- NICHD NIH HHS [R01 HD37486] Funding Source: Medline
- NIGMS NIH HHS [T32 GM07544] Funding Source: Medline
Ask authors/readers for more resources
Posterior prevalence is the general property attributed to HOX proteins describing the dominant effect of more posterior HOX proteins over the function of anterior orthologs in common areas of expression. To explore the HOX group 13 protein domains required for this property, we used the mouse Prx-1 promoter to drive transgenic expression of Hex constructs throughout the entire limb bud during development. This system allowed us to conclusively demonstrate a hierarchy of Hex function in developing limbs. Furthermore, by substituting the HOXD11 or HOXA9 homeodomain for that of HOXD 13, we show that a HOXD 13 homeodomain is not necessary for posterior prevalence. Proximal expression of these chimeric proteins unexpectedly caused defects consistent with wild-type HOXD13 mediated posterior prevalence. Moreover, group 13 non-homeodomain residues appear to confer the property as proximal expression of HOXA9 containing the HOXD 13 homeodomain did not result in limb reductions characteristic of HOXD13. These data are most compatible with models of posterior prevalence based on protein-protein interactions and support examination of the N-terminal non-homeodomain regions of Hex group 13 proteins as necessary agents for posterior prevalence. (c) 2006 Elsevier Inc. All rights reserved.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available