Journal
FEBS LETTERS
Volume 580, Issue 21, Pages 5084-5088Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.febslet.2006.08.034
Keywords
alcohol dehydrogenase; continuous culture; respiration; metabolic channelling; Zymomonas mobilis
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Perturbation of the aerobic steady-state in a chemostat culture of the ethanol-producing bacterium Zymoinonas mobilis with a small pulse of ethanol causes a burst of ethanol oxidation, although the reactant ratio of the alcohol dehydrogenase (ADH) reaction (INADHI[acetaidehyde][H+])/([ethanol][NAD(+)]) remains above the K, value. Simultaneous catalysis of ethanol synthesis and oxidation by the two ADH isoenzymes, residing in different redox microenvironments, has been proposed previously. In the present study, this hypothesis is verified by construction of an ADH-deficient strain and by demonstration that it lacks the oxidative burst in response to perturbation of its aerobic steady-state with ethanol. (c) 2006 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
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