Journal
VIROLOGY
Volume 353, Issue 2, Pages 333-343Publisher
ACADEMIC PRESS INC ELSEVIER SCIENCE
DOI: 10.1016/j.virol.2006.06.013
Keywords
fusion protein; hemagglutinin-neuraminidase protein; immunohistochemistry; in situ hybridization; phosphoprotein; reverse genetics; avian paramyxovirus; veterinary virology; emerging disease
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The effect of mutations of Newcastle disease virus (NDV) fusion (F) gene, hemagglutinin-neuraminidase (HN) gene, and phosphoprotein (P) gene and HN chimeras between the virulent Beaudette C and low virulence LaSota strains on pathogenesis and pathogenicity was examined in fully susceptible chickens. A virulent F cleavage site motif within a LaSota backbone increased pathogenicity and severity of clinical disease. A LaSota HN within a Beaudette C backbone decreased pathogenicity indices and disease severity. A Beaudette C HN within a LaSota backbone did not change either pathogenicity indices or severity of disease in chickens. Loss of glycosylation at site 4 of the HN or modified P gene of Beaudette C decreased pathogenicity indices and caused no overt clinicopathologic disease in chickens. Both pathogenicity indices and clinicopathologic examination demonstrated that the F, RN, and P genes of NDV collectively or individually can contribute to viral virulence. (c) 2006 Elsevier Inc. All rights reserved.
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