Journal
CEREBRAL CORTEX
Volume 16, Issue 10, Pages 1377-1388Publisher
OXFORD UNIV PRESS INC
DOI: 10.1093/cercor/bhj084
Keywords
corticogenesis; developing neocortex; GABA(A) receptor; GFP-MGE; telencephalic slice coculture
Categories
Funding
- NIMH NIH HHS [R01 MH069826] Funding Source: Medline
Ask authors/readers for more resources
During corticogenesis, cells from the medial ganglionic eminence (MGE) migrate tangentially into the neocortical anlage. Hare we report that gamma-aminobutyric acid (GABA), via GABA(A) receptors, regulates tangential migration. In embryonic telencephalic slices, bicuculline produced an outward current in migrating MGE-derived cells in the neocortex, suggesting the presence of and tonic activation by ambient GABA. Ambient GABA was also present in the MGE, although this required demonstration using as bioassay HEK293 cells expressing high-affinity alpha 6/beta 2/gamma 2s recombinant GABA(A) receptors. The concentration of ambient GABA was 0.5 +/- 0.1 mu M in both regions. MCE-derived cells before the corticostriate juncture (CSJ) were less responsive to GABA than those in the neocortex, and profiling of GABA(A) receptor subunit transcripts revealed different expression patterns in the MGE vis-a-vis the neocortex. These findings suggest a dynamic expression of GABA(A) receptor number or isoform as MGE-derived cells enter the neocortex and become tonically influenced by ambient GABA. Treatment with bicuculline or antibody against GABA did not affect migration of MGE-derived cells before the CSJ but decreased crossing index, reflecting impeded migration past the CSJ into the neocortex. Treatment with diazepam or addition of exogenous GABA increased crossing index. We conclude that ambient GABA promotes cortical entry of tangentially migrating MGE-derived cells.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available