Determination of poly(3-hydroxybntyrate) using a combination of enzyme-based biosensor and alkaline hydrolysis

The combination of an enzyme-based biosensor and alkaline hydrolysis was developed for the measurement of poly(3-hydroxybutyrate) (PHB). The principle of the determination is based on that the alkaline condition converts PHB to produce its monomer, 3-hydroxybutyrate (3-HB), which generates a detectable current signal by an amperometric biosensor through coupled two-enzyme reactions on an electrode. This method takes less than 40 min, and results in a linear detection range of 0.5 - 110 mg L-1 PHB with a detection limit of 0.3 mg L-1 by the saturated production of 3-HB; it can also take less than 15 min and result in a linear detection range of 1.0 - 160 mg L-1 PHB with a detection limit of 0.5 mg L-1 by a part production of 3-HB. The method also shows simple operation and high reproducibility.