Journal
ANALYTICAL CHEMISTRY
Volume 78, Issue 19, Pages 6948-6954Publisher
AMER CHEMICAL SOC
DOI: 10.1021/ac0613131
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Funding
- NIBIB NIH HHS [R01 EB005197-03, R01 EB005197, EB005197, R01 EB005197-01A1, R01 EB005197-02] Funding Source: Medline
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This paper demonstrates the ability to use capillary electrophoresis (CE) separation coupled with laser-induced fluorescence for analyzing the contents of single femtoliter-volume aqueous droplets. A single droplet was formed using a T-channel (3 mu m wide by 3 mu m tall) connected to microinjectors, and then the droplet was fluidically moved to an immiscible boundary that isolates the CE channel (50 Am wide by 50 Am tall) from the droplet generation region. Fusion of the aqueous droplet with the immiscible boundary effectively injects the droplet content into the separation channel. In addition to injecting the contents of droplets, we found aqueous samples can be introduced directly into the separation channel by reversibly penetrating and resealing the immiscible partition. Because droplet generation in channels requires hydrophobic surfaces, we have also investigated the advantages to using all hydrophobic channels versus channel systems with patterned hydrophobic and hydrophilic regions. To fabricate devices with patterned surface chemistry, we have developed a simple strategy based on differential wetting to deposit selectively a hydrophilic polymer (poly(styrenesulfonate)) onto desired regions of the microfluidic chip. Finally, we applied our device to the separation of a simple mixture of fluorescein- labeled amino acids contained within a similar to 10- L droplet.
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