Apparent delay of product secretion by product adsorption in Aspergillus niger

Optimization and modeling of cultivation processes for the production of heterologous and homologous proteins requires a comprehensive characterization of product formation. Methods have been developed for the experimental investigation of mass transport, substrate turnover and product formation in biopellets of the fungus Aspergillus niger for the production of glucoamylase (GAM). GAM production in A. niger was chosen as a model system, its advantage being its potential for complex, posttranslational protein modifications.