Journal
BIOTECHNOLOGY LETTERS
Volume 28, Issue 19, Pages 1527-1535Publisher
SPRINGER
DOI: 10.1007/s10529-006-9122-7
Keywords
lactate; betaine; E-coli; methylglyoxal; mgsA; polylactic acid
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Two new strains of Escherichia coli B were engineered for the production of lactate with no detectable chiral impurity. All chiral impurities were eliminated by deleting the synthase gene (msgA) that converts dihydroxyacetonephosphate to methylglyoxal, a precursor for both L(+)- and D(-)- lactate. Strain TG113 contains only native genes and produced optically pure D(-)- lactate. Strain TG108 contains the ldhL gene from Pediococcus acidilactici and produced only L(+)- lactate. In mineral salts medium containing 1 mM betaine, both strains produced over 115 g (1.3 mol) lactate from 12% (w/v) glucose, > 95% theoretical yield.
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