Journal
EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS
Volume 35, Issue 8, Pages 709-712Publisher
SPRINGER
DOI: 10.1007/s00249-006-0070-x
Keywords
GPCR; neurotensin receptor; surface plasmon resonance; ligand-binding; high-throughput screening
Categories
Funding
- Medical Research Council [G0400503B] Funding Source: researchfish
Ask authors/readers for more resources
Despite their clinical importance, detailed analysis of ligand binding at G-protein coupled receptors (GPCRs) has proved difficult. Here we successfully measure the binding of a GPCR, neurotensin receptor-1 (NTS-1), to its ligand, neurotensin (NT), using surface plasmon resonance (SPR). Specific responses were observed between NT and purified, detergent-solublised, recombinant NTS-1, using a novel configuration where the biotinylated NT ligand was immobilised on the biosensor surface. This SPR approach shows promise as a generic approach for the study of ligand interactions with other suitable GPCRs.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available