Journal
PLANT CELL
Volume 18, Issue 10, Pages 2431-2442Publisher
AMER SOC PLANT BIOLOGISTS
DOI: 10.1105/tpc.106.045088
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DNA replication in cycling eukaryotic cells necessitates the reestablishment of chromatin after nucleosome redistribution from the parental to the two daughter DNA strands. Chromatin assembly factor 1 ( CAF-1), a heterotrimeric complex consisting of three subunits ( p150/ p60/ p48), is one of the replication- coupled assembly factors involved in the reconstitution of S- phase chromatin. CAF- 1 is required in vitro for nucleosome assembly onto newly replicated chromatin in human cells and Arabidopsis thaliana, anddefects in yeast ( Saccharomycescerevisiae) affectDNAdamagerepair processes, predominantly thoseinvolvedin genome stability. However, in vivo chromatin defects of caf- 1 mutants in higher eukaryotes are poorly characterized. Here, we show that fasciata1- 4 ( fas1- 4), a new allele of the Arabidopsis fas1 mutant defective in the p150 subunit of CAF- 1, has a severe developmentalphenotype, reduced heterochromatin content, andamoreopenconformation ofeuchromatin. Mostimportantly, homologous recombination ( HR), a process involved in maintaining genome stability, is increased dramatically in fas1- 4, as indicated by a 96- fold stimulation of intrachromosomal HR. Together with the open conformation of chromatin and the nearly normal expression levels ofHRgenes in the mutant, this result suggests that chromatin is a major factor restrictingHRin plants.
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