Journal
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
Volume 1758, Issue 10, Pages 1541-1556Publisher
ELSEVIER SCIENCE BV
DOI: 10.1016/j.bbamem.2006.05.019
Keywords
LAURDAN; cholesterol; two photon excitation fluorescence microscopy; giant vesicle; lipid phase coexistence; raft
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In the last few years several experimental strategies based on epi-, confocal and two photon excitation fluorescence microscopy techniques have been employed to study the lateral structure of membranes using giant vesicles as model systems. This review article discusses the methodological aspects of the aforementioned experimental approaches, particularly stressing the information obtained by the use of UV excited fluorescent probes using two-photon excitation fluorescence microscopy. Additionally, the advantages of utilizing visual information, to correlate the lateral structure of compositionally simple membranes with complex situations, i.e., biological membranes, will be addressed. (c) 2006 Elsevier B.V. All rights reserved.
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