Journal
BIOMEDICAL CHROMATOGRAPHY
Volume 20, Issue 10, Pages 981-984Publisher
WILEY
DOI: 10.1002/bmc.676
Keywords
astaxanthin; shrimp waste; high-performance liquid chromatography (HPLC); lactic fermentation
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In the present study, a simple and rapid reversed-phase HPLC method for the determination of astaxanthin in shrimp waste hydrolysate has been developed and validated. The analytical procedure involves the direct extraction of astaxanthin from the lipid fraction with methanol. The analytical column, SS Exil ODS, was operated at 25 degrees C. The mobile phase consisted of a mixture of water:methanol:dichloromethane:acetonitrile (4.5:28:22:45.5 v/v/v/v) at a flow rate of 1.0 mL/min. Detection and identification were performed using a photodiode array detector (lambda(detection) = 476 nm). The proposed HPLC method showed adequate linearity, repeatability and accuracy. Copyright (c) 2006 John Wiley & Sons, Ltd.
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