Journal
ANNALS OF BIOMEDICAL ENGINEERING
Volume 40, Issue 6, Pages 1268-1276Publisher
SPRINGER
DOI: 10.1007/s10439-012-0515-6
Keywords
Microfluidics; Spinal cord injury; Axonal regeneration
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Funding
- Graduate Studies Abroad Fellowship [KRF-2005-215-D00030]
- WCU (World Class University) through Korea Research Foundation
- Ministry of Education, Science and Technology [R31-2008-000-10083-0]
- Pioneer Research Center [20110001643]
- Biomembrane Plasricity Research Center through National Research Foundation (NRF)
- National Research Foundation (NRF)
- Ministry of Education, Science and Technology
- Ministry of Knowledge Economy (MKE) of Korea [10033657]
- National Research Foundation of Korea [과C6A1803, R31-2012-000-10083-0, 2010-0029525] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
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We describe the development of experimental platforms to quantify the regeneration of injured central nervous system (CNS) neurons by combining engineering technologies and primary neuronal cultures. Although the regeneration of CNS neurons is an important area of research, there are no currently available methods to screen for drugs. Conventional tissue culture based on Petri dish does not provide controlled microenvironment for the neurons and only provide qualitative information. In this review, we introduced the recent advances to generate in vitro model system that is capable of mimicking the niche of CNS injury and regeneration and also of testing candidate drugs. We reconstructed the microenvironment of the regeneration of CNS neurons after injury to provide as in vivo like model system where the soluble and surface bounded inhibitors for regeneration are presented in physiologically relevant manner using microfluidics and surface patterning methods. The ability to control factors and also to monitor them using live cell imaging allowed us to develop quantitative assays that can be used to compare various drug candidates and also to understand the basic mechanism behind nerve regeneration after injury.
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