Precise centromere mapping using a combination of repeat junction markers and chromatin immunoprecipitation-polymerase chain reaction

Centromeres are difficult to map even in species where genetic resolution is excellent. Here we show that functions between repeats provide reliable single-copy markets for recombinant inbred mapping within centromeres and pericentromeric heterochromatin. Repeat function mapping was combined will anti-CENH3-mediated ChIP to provide a definitive map position for maize centromere 8.