Journal
INTERNATIONAL BIODETERIORATION & BIODEGRADATION
Volume 58, Issue 3-4, Pages 203-208Publisher
ELSEVIER SCI LTD
DOI: 10.1016/j.ibiod.2006.06.010
Keywords
polychlorobiphenyl; herbicide; Burkholderia LB400; glutathione S-transferase; bioremediation
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The bphK gene located in the bph operon of Burkholderia LB400 encodes a protein, BphK(LB400), with significant sequence similarity to glutathione-S-transferases (GST), a group of enzymes involved in the detoxification of many endobiotic and xenobiotic substances. Comparison of the amino acid sequence of BphK(LB400) with GST from other polychlorinated biphenyl (PCB)-degrading bacteria identified a number of highly conserved amino acids in the C-terminal region of the protein that may be associated with substrate specificity. In this study, two of these conserved amino acids in BphK(LB400) (amino acids 152 and 180) were selected for mutation, using site-directed mutagenesis, and substrate specificity assays. BphK(LB400) (wildtype and mutant) was over-expressed in Escherichia coli where the bphK gene (wildtype and mutant) is under the expression of a hie promoter and is induced by isopropyl thiogalactoside, and bacterial cell extracts were prepared for GST activity assays. Mutations at amino acids 152 and 180 were shown to affect GST activity of BphK(LB400) using 1-chloro-2,4-dinitrobenzene, the model substrate for GST activity assays; 4-chlorobenzoate and 3-chlorobenzoate, intermediates in the polychlorinated biphenyl (PCB) degradation pathway, and 2,4-dichlorophenoxyacetate and atrazine, commonly used herbicides; as substrates. A BphK(LB400) mutant (Ala180Pro) is identified in this study as having increased activity towards all substrates tested. This mutant may have potential in bioremediation. (c) 2006 Elsevier Ltd. All rights reserved.
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